Here we present the 6-second resolution raw TSG data collected during the The South Atlantic Meridional Overturning Circulation Basin-wide Array (SAMBA) Monitoring Line cruise, which was conducted on the Algoa Voyage 285 between 27 September 2022 and 07 October 2022. A SeaBird SBE45 Thermosalinograph (TSG) is used to opportunistically collect underway near-surface temperature and conductivity measurements during research and monitoring cruises. Water is continuously pumped to the TSG from an intake located in the hull of the vessel, and the observations are continuously interfaced with navigational information. A temperature sensor close to the intake provides temperature measurements of the incoming water (T1). The temperature of the water inside the conductivity cell (T2) is used to accurately compute salinity (S) from the conductivity measurements (C). The cruise operated from Slangkop, off Cape Town, westwards to 11° E, in the South Atlantic Ocean along the SAMBA transect and south of Hondeklipbaai on the West coast. The objectives of the cruise were to recover an Acoustic Doppler Current Profiler (ADCP) mooring (M4) along the Integrated Ecosystem Programme (IEP)’s Scarborough Monitoring line to collect current data on the Benguela Jet Current; recover 3 x tall moorings on the SAMBA transect (M8; M9 & M10), incorporating ADCP’s and Sea-Bird Microcats; complete 21 Conductivity, Temperature, Depth (CTD) casts to a maximum depth of ~1200m (using Hydro winch) along the SAMBA transect; recover; service and re-deploy 1 TRAFFIC sediment trap mooring (SBUS East-03), south of Hondeklipbaai; undertake Chlorophyll and Oxygen sampling at CTD stations and underway along SAMBA transect; collect underway Thermosalinograph (TSG); Surface Photosynthetically Active Radiation (SPAR) and Ship-mounted Acoustic Doppler Current Profilers (S-ADCP) data and provide training to newly employed Department of Forestry, Fisheries and the Environment (DFFE) personnel and interns.